{"id":49502,"date":"2021-05-18T17:41:09","date_gmt":"2021-05-18T12:11:09","guid":{"rendered":"https:\/\/newsmakhani.com\/?p=49502"},"modified":"2021-05-18T17:41:09","modified_gmt":"2021-05-18T12:11:09","slug":"dst-institute-develops-new-multiplex-rt-pcr-kit-with-novel-gene-targets-to-facilitate-detection-across-various-mutant-strains-of-covid-19","status":"publish","type":"post","link":"https:\/\/newsnarad.com\/pa\/dst-institute-develops-new-multiplex-rt-pcr-kit-with-novel-gene-targets-to-facilitate-detection-across-various-mutant-strains-of-covid-19\/","title":{"rendered":"DST institute develops new multiplex RT-PCR kit with novel gene targets to facilitate detection across various mutant strains of COVID 19"},"content":{"rendered":"

\u0a2e\u0a3e\u0a2b\u0a3c \u0a15\u0a30\u0a28\u0a3e, \u0a07\u0a39 \u0a16\u0a2c\u0a30 \u0a24\u0a41\u0a39\u0a3e\u0a21\u0a40 \u0a2c\u0a47\u0a28\u0a24\u0a40 \u0a2d\u0a3e\u0a38\u0a3c\u0a3e \u0a35\u0a3f\u0a71\u0a1a \u0a09\u0a2a\u0a32\u0a2c\u0a27 \u0a28\u0a39\u0a40\u0a02 \u0a39\u0a48\u0964 \u0a15\u0a3f\u0a30\u0a2a\u0a3e \u0a15\u0a30\u0a15\u0a47 \u0a07\u0a71\u0a25\u0a47 \u0a26\u0a47\u0a16\u0a4b\u0964<\/a><\/p>

<\/p>\n

This unique RT-PCR kit will be a significant weapon in our fight against COVID-19 by a facile detection of SARS-CoV-2 mutations: Secretary, DST, Prof Ashutosh Sharma<\/span><\/h4>\n

DELHI MAY 18, 2021:<\/strong><\/p>\n

A newly developed\u00a0multiplex RT-PCR kit<\/strong>\u00a0has a higher accuracy of detecting covid19 across the various mutant strains of the virus responsible for the global pandemic.<\/p>\n

As the pandemic is going through a second wave with multiple variants, the selection of target genes in multiplex RT-PCR assay is becoming critical for accurate detection of the virus.<\/p>\n

Even though coronaviruses make far fewer errors than other RNA viruses, the mutations in S, R, and N genes often interfere with RT-PCR assay.\u00a0 For example, the \u201cvariant of concern\u201d B1.1.7 (also known as the UK variant) has a 69-70del,\u00a0due to deletion of 6 bases in the RNA, which resulted in S gene drop out from RT-PCR assay.<\/p>\n

The new multiplex RT-PCR kit developed\u00a0Sree Chitra Tirunal Institute for Medical Sciences and Technology<\/strong>\u00a0(SCTIMST<\/strong>), an Institute of National Importance under the\u00a0Department of Science and Technology<\/strong>, Government of India targets two SARS CoV2 genes: RdRp and ORFb-nsp14, and the human RNAse P gene as the internal control to help detect a range of mutant strains.<\/p>\n

Various studies have shown that RdRp and ORF1b-nsp14 genes are more sensitive in detecting Covid19. In order to target the multiple variants in the second wave, using two highly accurate confirmatory genes like RdRp and ORF-nsp14, can give precise results. The ORFb-nsp14 is one of the least mutated genes in Covid19 and currently, there are no kits in the market with ORF-nsp14 as the target.<\/p>\n

The new kit is based on multiplex Taqman chemistry, amplifying all three genes in a single reaction. The amplification time for the assay is 45 minutes, apart from the time required for the RNA isolation from nasopharyngeal swab samples. Multiplexing two confirmatory genes will help shortlist possible new variants if one of the genes fails to amplify and can be marked for sequence analysis.<\/p>\n

ICMR<\/strong>\u00a0has validated this kit at the\u00a0National Institute of Virology<\/strong>, Pune, and found that it has 97.3% sensitivity and 100% specificity in covid19 detection.<\/p>\n

SCTIMST has signed a non-exclusive license MoU with Huwel Lifesciences, Hyderabad, on 14th May 2021 to commercialize the kit.<\/p>\n

\u201cThis unique RT-PCR kit will be a significant weapon in our fight against COVID-19 by a facile detection of SARS-CoV-2 mutations which are becoming increasingly important,\u201d said Secretary, DST, Prof Ashutosh Sharma.<\/p>","protected":false},"excerpt":{"rendered":"

\u0a2e\u0a3e\u0a2b\u0a3c \u0a15\u0a30\u0a28\u0a3e, \u0a07\u0a39 \u0a16\u0a2c\u0a30 \u0a24\u0a41\u0a39\u0a3e\u0a21\u0a40 \u0a2c\u0a47\u0a28\u0a24\u0a40 \u0a2d\u0a3e\u0a38\u0a3c\u0a3e \u0a35\u0a3f\u0a71\u0a1a \u0a09\u0a2a\u0a32\u0a2c\u0a27 \u0a28\u0a39\u0a40\u0a02 \u0a39\u0a48\u0964 \u0a15\u0a3f\u0a30\u0a2a\u0a3e \u0a15\u0a30\u0a15\u0a47 \u0a07\u0a71\u0a25\u0a47 \u0a26\u0a47\u0a16\u0a4b\u0964 This unique RT-PCR kit will be a significant weapon in our fight against COVID-19 by a facile detection of SARS-CoV-2 mutations: Secretary, DST, Prof Ashutosh Sharma DELHI MAY 18, 2021: A newly developed\u00a0multiplex RT-PCR kit\u00a0has a higher accuracy of detecting […]<\/p>\n","protected":false},"author":10,"featured_media":49505,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1528,159,1563,122],"tags":[23962,24987,24988],"class_list":["post-49502","post","type-post","status-publish","format-standard","has-post-thumbnail","category-covid-19","category-delhi","category-health","category-news","tag-covid-19-test","tag-dst-institute-develops","tag-new-multiplex-rt-pcr-kit"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/newsnarad.com\/pa\/wp-json\/wp\/v2\/posts\/49502","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/newsnarad.com\/pa\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/newsnarad.com\/pa\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/newsnarad.com\/pa\/wp-json\/wp\/v2\/users\/10"}],"replies":[{"embeddable":true,"href":"https:\/\/newsnarad.com\/pa\/wp-json\/wp\/v2\/comments?post=49502"}],"version-history":[{"count":0,"href":"https:\/\/newsnarad.com\/pa\/wp-json\/wp\/v2\/posts\/49502\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/newsnarad.com\/pa\/wp-json\/wp\/v2\/media\/49505"}],"wp:attachment":[{"href":"https:\/\/newsnarad.com\/pa\/wp-json\/wp\/v2\/media?parent=49502"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/newsnarad.com\/pa\/wp-json\/wp\/v2\/categories?post=49502"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/newsnarad.com\/pa\/wp-json\/wp\/v2\/tags?post=49502"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}